编号\r
北京华越洋生物VECT6033\r
pCMV-‐Tag\r 4A载体基本信息
载体名称: 质粒类型: 高拷贝/低拷贝: 克隆方法: 启动子: 载体大小: 5' 测序引物及序列: 3' 测序引物及序列:
载体标签: 载体抗性: 筛选标记: 克隆菌株: 宿主细胞（系）:
备注: 稳定性: 组成型/诱导型: 病毒/非病毒:
pCMV-Tag 4A
载体名称\r
pCMV-‐Tag\r 4A\r
哺乳动物表达载体 高拷贝
多克隆位点；限制性内切酶 CMV 4319 bp
CMV Forward: CGCAAATGGGCGGTAGGCGTG
T7
FLAG tag (C-末端）
卡那霉素 Neomycin XL1-Blue --
FLAG标签含8个氨基酸残基(DYKDDDDK)；
pCMV-Tag 4 A,B,C的差别仅在于读码框（阅读框）处。 瞬表达 组成型 非病毒
pCMV-‐Tag\r 4A载体质粒图谱和多克隆位点信息
pCMV-‐Tag\r 4A载体简介\r \r
ADDITIONAL\r MATERIALS\r REQUIRED\r T4\r DNA\r ligase\r
Taq\r DNA\r polymerase\r
Taq\r DNA\r polymerase\r buffer\r TE\r buffer\r CIAP\r
Falcon\r 2059\r polypropylene\r tubes\r (15-‐ml)\r Water\r baths\r (37°C\r and\r 42°C)\r \r
XL1-‐Blue\r Genotype:\r recA1\r endA1\r gyrA96\r thi-‐1\r hsdR17\r supE44\r relA1\r lac\r [F´\r proAB\r lacIqZΔM15\r Tn10\r (Tetr)].\r
The\r XL1-‐Blue\r cells\r supplied\r with\r the\r pCMV-‐Tag\r vectors\r are\r not\r competent\r cells.\r Refer\r to\r Hanahan\r (1983)\r for\r a\r protocol\r for\r producing\r competent\r cells.\r \r
pCMV-‐Tag\r 4A载体序列
ORIGIN
1 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA 61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG 121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG 181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA 241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC 301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC 361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC 421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA 481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG 541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA 601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC 661 GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC 721 TTATCGATAC CGTCGACCTC GAGGATTACA AGGATGACGA CGATAAGTAG GGCCCGGTAC 781 CTTAATTAAT TAAGGTACCA GGTAAGTGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC 841 AATTCACTCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAGATCCAAT 901 TTTTAAGTGT ATAATGTGTT AAACTACTGA TTCTAATTGT TTGTGTATTT TAGATTCACA 961 GTCCCAAGGC TCATTTCAGG CCCCTCAGTC CTCACAGTCT GTTCATGATC ATAATCAGCC 1021 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC 1081 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT 1141 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 1201 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT 1261 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA 1321 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA 1381 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC 1441 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG 1501 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG 1561 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG 1621 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG 1681 CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 1741 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 1801 CTTCAATAAT ATTGAAAAAG GAAGAATCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT 1861 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 1921 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 1981 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 2041 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 2101 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 2161 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC 2221 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 2281 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 2341 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 2401 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 2461 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 2521 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 2581 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
2641 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 2701 GAACATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC 2761 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 2821 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC 2881 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAACTTGGCG GCGAATGGGC TGACCGCTTC 2941 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 3001 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 3061 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 3121 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 3181 CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG 3241 CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC 3301 GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC 3361 AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG 3421 GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC 3481 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 3541 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 3601 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 3661 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 3721 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT 3781 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 3841 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 3901 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 3961 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 4021 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 4081 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 4141 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 4201 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 4261 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCC //
\r
其他哺乳动物表达载体：\r
pCHO1.0 pcDNA3.1/His C pcDNA3.1/His B pcDNA3.1/His A pIRESpuro3 pIRES2-EGFP pTT5
pNFkB-DD-tdTomato pBI-CMV5 pSEAP2-Basic pSEAP2-Control
pBApo-CMV-Pur
pcDNA5/FRT/V5-His-TOPO pcDNA5/FRT/TO-TOPO pcDNA5/TO pcDNA5/FRT/TO pcDNA5/FRT pFLAG-CMV2
pcDNA3.1/CT-GFP-TOPO pcDNA3.1/NT-GFP-TOPO pOptiVEC-TOPO pCMV-MEKK1
pOPRSVI pREP4 pDual-GC pBK-RSV pBK-CMV pBI-CMV4
pcDNA4/TO/Myc-His/LacZ pOPI3CAT pGene/V5-His B pSwitch pCMVLacI
pBI-CMV3 pBI-CMV2 pBI-CMV1
pNFκB-MetLuc2-Reporter pCRE-MetLuc2-Reporter pAcGFP1-Actin
pAcGFP1-N In-Fusion Ready pAcGFP1-C3 pAcGFP1-C pAcGFP1-p53 pAcGFP1-Mito pAcGFP1-Mem pAcGFP1-Lam pAcGFP1-Golgi pAcGFP1-F pAcGFP1-Hyg-C1 pAsRed2-N1 ptdTomato-N1 pCMV-tdTomato pCRE-DD-tdTomato pCMV-DsRed-Express2 pEF1α-tdTomato pCRE-hrGFP ptdTomato-C1 pAsRed2-C1 pGL3-Promoter pGL3 basic pAcGFP1-C2 pAcGFP1-C1 pAcGFP1-N3 pAcGFP1-N2 pAcGFP1-N1
pAcGFP1-C In-Fusion Ready pCRE-DD-AmCyan1 pNFkB-DD-AmCyan1 pDsRed2-Bid pDsRED2-Mito
pDD-AmCyan1 Reporter pAmCyan1-N1 pAmCyan1-C1
pEF1α-IRES-DsRed-Express2 pEF1α-DsRed-Monomer-N1 pDsRED-Monomer-N1 pDsRed-Express-N1
pCMV-MEK1 pCMV-PKA
pcDNA6.2/nTC-Tag-DEST pcDNA6.2/cTC-Tag-DEST pcDNA3.2/V5/GW/D-TOPO pcDNA6.2/V5/GW/D-TOPO
pcDNA6.2/nGeneBLAzer-GW/D-TOPO pcDNA6.2/C-YFP-DEST pcDNA6.2/cGeneBLAzer-DEST pcDNA6/V5-His A pcDNA6/V5-His B pcDNA6/V5-His C pcDNA6/myc-His C pcDNA6/myc-His A pcDNA6/myc-His B
pcDNA6.2/nGeneBLAzer-DEST pcDNA4/HisMax-TOPO pcDNA6.2/nLumio-DEST pcDNA6.2/cLumio-DEST pcDNA4/myc-His C pcDNA4/HisMax C pcDNA4/HisMax A c-Flag pcDNA3 pcDNA4/HisMax B pcDNA4/myc-His B pcDNA4/myc-His A pcDNA4/His C pcDNA4/His B pcDNA4/His A pcDNA6/TR
pcDNA4/TO/Myc-His A pcDNA4/TO
pcDNA4/TO/Myc-His B pcDNA4/TO/Myc-His C pcDNA3.3-TOPO pBudCE4.1 pFLAG-CMV-4 pFLAG-CMV-3 pFLAG-CMV-2 pFLAG-CMV-5a p3XFLAG-CMV-9 p3xFLAG-CMV-10 p3XFLAG-CMV-8 p3XFLAG-CMV-7.1
pVgRxR pIND pTRE3G-Luc pTRE3G pTRE2-hygro pTRE-Tight pTK-hyg pTet-On pTet-Off
pTet on advanced pRevTRE pRevTet-On pRevTet-Off pCMV-Tet3G pTRE2 pBD-NF-κB pCMV-AD pCMV-BD pBIND-Id Control pBIND pG5 luciferase pACT-MyoD pACT
pCMV-SPORT6 pGL4.13 pGL4.19 pGL4.26 pGL4.20 pGL4.29 pGL4.30 pGL4.27 pGL4.75 pGL4.10 pGRN145 pSecTag2 A pEBVHis B pEBVHis A pCMV-Tag 3C pCMV-Tag 3A pCMV-Tag 3B pCMV-Tag 5C pCMV-Tag 5A pCMV-Tag 4A pCMV-Tag 5B
p3XFLAG-CMV-7 pDsRed-Express-C1 pIRES2-ZsGreen1 pDsRed-Express2-C1 pDsRed-Express2-N1 pEF1α-DsRed-Express2 pIRES2-DsRed-Express pIRES2-DsRed-Express2 pIRES-hrGFP-1a pIRESneo2 pIRESneo pIREShyg3
\r
pDsRed-Monomer-N In-Fusion Ready p3XFLAG-CMV-13 p3XFLAG-CMV-14 plRES2-ZsGreen1 pBApo-EF1α-pur pBApo-EF1α-neo pBApo-CMV pBApo-CMV-neo pIRES-EGFP pIRESneo3 pDsRed-Monomer pIRES
pCMV-Tag 4B pCMV-Tag 2C pCMV-Tag 2B pCMV-Tag 2A pCMV-LacZ pCMV-Myc
pEF1α-IRES-AcGFP1 pEF1α-IRES-ZsGreen1 pEF1α-AcGFP1-N1 pIRES2-DsRed2 pIRES2-AcGFP1
因篇幅问题不能全部显示，请点此查看更多更全内容