测序通用引物序列

常见载体的测序引物： Primer of Vector:

Vector：Primer(F)；Primer(R) pACT T7 T3

pACT2 GAL4 AD pACT2-R pAS2-1 GAL4 BD pAS2-1.R pB42AD pB42ADF pB42ADR pBACPAK8 BAC1 BAC2 pBK-CMV T7 T3

PBS(SK/KS)/M13- M13F/T7 T3/M13R pBV220 PBV220F PBV220R pCAMBIA 1301(1300) P1 P2

pCAMBIA 2300 M13R(-48) M13F(-47) PCANTB5E S1/M13R S6

pCAT3-enhancer RVP3 此载体无反向引物 pcDNA3.0 CMV-F/T7 SP6/BGH pcDNA3.1 T7 BGH

pcDNA4 T7/CMV-F BGH

pcDNA6 T7/CMV-F(J21025在T7前面） BGH pcDNAII T7 SP6 pCE2.1 M13F M13R pCEP4 pCEP-F EBV-R pCF-T M13F M13R

pCI T7（17Base） 此载体无反向引物 pCI-neo T7（17Base） T3 pCMS-EGFP T7 T3

pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F pCMV5R

pCMV5-Flag pCMV5F pCMV5R pCMV-MYC/HA pCMV-F pCMV-R pCMV-Sport M13F/T7 SP6/M13R pCMV-Tag(KAN+) T3 T7

pCR2.1-TOPO M13F/T7 M13R pCR3.1 T7 BGH pCS2 SP6 T7

pDonar M13F M13R pDONR221 M13F M13R pDrive T7 SP6

pDRIveR(KAN+) T7 SP6

pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’ pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-R pECFP-C pEGFP-C-5’ pECFP-C-3′

pECFP-N1 pEGFP-N-5’ pEGFP-N-3’ pEF/myc/ER pEF-F pCDNA3.1R

pEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3′ pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO(KAN+) M13F M13R pET-*(His)(KAN+) T7 T7 Ter pET22(a,b,c)(AMP+) T7 T7 Ter pET28,30,24,49(KAN+) T7 T7 Ter pET32(a,b,c)(AMP+) T7/S.tag T7 Ter pET50(amp+) T7/s.tag T7TER pET44(AMP+) T7/s.tag T7 Ter

pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pEYFP-C1 pEGFP-C-5’ pEGFP-C-3′

pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3′AD pGADGH GAL4 AD 3′AD

pGADT7-Rec GAL4 AD/T7 3′AD pGAPZa-A a-FACTOR 3′AOX pGBKT7(Kana+) T7 3′BD

pGEM-T(-Easy) M13F/T7 SP6/M13R pGEX-(*)T pGEX-4T-5’ pGEX-4T-3’ pGL2 GLP1 GLP2

pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2) PinpointTM Vector Pinpoint Primer SP6 pIRES PIRES-F/T7 T3/PIRES-R (J40720)

pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLXSN pLXSN-F pLXSN-R

pMAL-c2E MalE primer M13F(-47)

pMAL-C2x P5’ P3’

pMAL-p2X MalE primer M13F(-47)

PMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48)

pPIC9K(AMP+、ZEO+） 5’AOX/a-factor 3’AOX pPICZa 5′AOX/PPICZa-F 3′AOX

pPROEXHTA M13R(-48) 无反向引物 pQE30or40 pQE30-F pQE-R: pRSET T7 T7TER

pSilencer3.1-H1 hygro M13F(-47) 3.0REV pSILENCEV2.0-U6 T7 2.0REV pSK01-T M13F M13R pSK-CMV(KAN+) T7 T3 pSP72 T7 SP6

pSport1 SP6/M13F(-47) T7/M13R pSUPER T7 T3

pTA2 M13F/T7(优先使用M13F） T3/M13R pT-Adv M13F /T7 M13R

pTARGET TM pTarget.F(在T7前面）/T7 pTarget.R pTO-T7 T7 T7TER

pTRC99a-c pTRC99C-F pTRC99C-R pTriPLEx2 5′pTriPLEx2 T7

pTWIN-1 T7 T7TER(客户确认再用）

pUC18(19)/118(119) M13F(-47) M13R(-48) pUCm-T M13F/T7 M13R pVAX1 T7 BGH

pWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48) pXT7 T7 SP6

pYES2 T7 pYes2.R

pLEXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-R

pRECEIVER CMV-F 此载体无反向引物

pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSP64/65 SP6 此载体无反向引物 pSTBlue-1 T7 SP6

pT7Blue(R) T7 M13F(-47)

引物名称 序列(5′-3′)：

M13R：CAG GAA ACA GCT ATG ACC M13F：TGT AAA ACG ACG GCC AGT

M13F(-47)：CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48)：AGC GGA TAA CAA TTT CAC ACA GGA M13(-96)：CCC TCA TAG TTA GCG TAA CG SP6：ATT TAG GTG ACA CTA TAG T7：TAA TAC GAC TCA CTA TAG GG

T7 terminator：TGC TAG TTA TTG CTC AGC GG T3：ATT AAC CCT CAC TAA AGG GA

pGEX-4T-5′：GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3′：CCG GGA GCT GCA TGT GTC AGA GG GLp1：TGT ATC TTA TGG TAC TGT AAC TG GLp2：CTT TAT GTT TTT GGC GTC TTC CA RVp3：CTA GCA AAA TAG GCT GTC CC RVp4：GAC GAT AGT CAT GCC CCG CG pcDNA3.1R：TAG AAG GCA CAG TCG AGG

PinPoint primer： CGT GAC GCG GTG CAG GGC G

pCMV-F： TCT AAA AGC TGC GGA ATT GT pCMV-R： TCCAAACTCATCAATGTATC

pTRC99C-F: TTG CGC CGA CAT CAT AAC pTRC99C-R: CTGCGTTCTGATTTAATCTG pCEP-F: AGA GCT CGT TTA GTG AAC CG EBV-R : GTG GTT TGT CCA AAC TCA TC

pIRES2-EGFP.P5’: GTA GGC GTG TAC GGT GGG AG pIRES2-EGFP.P3’: AAC GCA CAC CGG CCT TAT TC 3′AD: AGA TGG TGC ACG ATG CAC AG

CMV -F： CGC AAA TGG GCG GTA GGC GTG S1： CAA CGT GAA AAA ATT ATT ATT CGC S6： GTA AAT GAA TTT TCT GTA GTA GG 5`AOX1：GAC TGG TTC CAA TTG ACA AGC 3`AOX1：GCA AAT GGC ATT CTG ACA TCC α-Factor：TAC TAT TGC CAG CAT TGC TGC GAL4 AD：TAC CAC TAC AAT GGA TG pACT2-R：GTGCACGATGCACAGTTGAA

pB42ADF：CCA GCC TCT TGC TGA GTG GAG ATG pB42ADR：AAG CCG ACA ACC TTG ATT GGA G pEGFP-N-5’：TGG GAG GTC TAT ATA AGC AGA G pEGFP-N-3’：CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5’： CAT GGT CCT GCT GGA GTT CGT G pEGFP-C-3′ ：TAT GGC TGA TTA TGA TCA GT PBV220F：AAG AAG GGC AGC ATT CAA AG PBV220R：CTG CGT TCT GAT TTA ATC TG U6：ATG GAC TAT CAT ATG CTT ACC GTA 2.0rev primer：AGG CGA TTA AGT TGG GTA 3.0rev：GAG TTA GCT CAC TCA TTA GGC S.tag：GAA CGC CAG CAC ATG GAC

5′TriplEx2：CTC CGA GAT CTG GAC GAG C RVP4：GAC GAT AGT CAT GCC CCG CG RVP3：CTA GCA AAA TAG GCT GTC CC pQE30-R：GTT CTG AGG TCA TTA CTG G pQE30-F：TGA GCG GAT AAC AAT TTC AC pEF-F：TCA AGC CTC AGA CAG TGG TTC

pDSRED-N1-R：TGA AGC GCA TGA ACT CCT TG

pDsRED-express-C1-F：TCC CAC AAC GAG GAC TAC AC pCMV5R： ATT ATA GAG GAC ACC TAG TC pCMV5F： TTC CAA AAT GTC GTA ATA AC P5′： TGC GTA CTG CGG TGA TCA AC P3′： CTG CAA GGC GAT TAA GTT GG BAC2： ACG CAC AGA ATC TAG CGC TT BAC1： AAC CAT CTC GCA AAT AAA TA

3′BD： TAA GAG TCA CTT TAA AAT TTG TAT AC pTarget.F： CCA GGA TTT TCC CAG TCA C pTarget.R： GGC TTT ACA CTT TAT GCT TC

T7(17base)： ACA TCC ACT TTG CCT TTC TC pYes2.R： TCG GTT AGA GCG GAT GTG GAL4 BD： TCA TCG GAA GAG AGT AG

pAS2-1.R： AAG AGT TAC TCA AGA ACA AGA A pFlag-CMV-F: GGT AGG CGT GTA CGG TGG pFlag-CMV-R: GCA CTG GAG TGG CAA CTT pIRES-F: CTT ACT GAC ATC CAC TTT GC pIRES.R: CAC TGC ATT CTA GTT GTG GT P1： CCA GGC TTT ACA CTT TAT GC P2： GCG ATT AAG TTG GGT AAC GC

pLEXA-F： CGT CAG CAG AGC TTC ACC AT pLEXA-R： TAA AAC CTA AGA GTC ACT TT

pLNCX-F： AGC TCG TTT AGT GAA CCG TCA GAT CG pLNCX-R： ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F： CTT GAA CCT CCT CGT TCG AC pLXSN-R： GTT GCT GAC TAA TTG AGA TG

pSHUTTLE-CMV-F： GGT CTA TAT AAG CAG AGG TG

pSHUTTLE-CMV-R： GTG GTA TGG CTG ATT ATG ATC AG MalE Primer： GGT CGT CAG ACT GTC GAT GAA GCC

引物名称 M13R M13F M13F(-47) M13R(-48) M13(-96) SP6 T7 T7 terminator T3 pGEX-4T-5' pGEX-4T-3' GLp1 GLp2 RVp3 RVp4 pcDNA3.1R PinPoint primer pCMV-F 序列(5'-3') CAG GAA ACA GCT ATG ACC TGT AAA ACG ACG GCC AGT CGC CAG GGT TTT CCC AGT CAC GAC AGC GGA TAA CAA TTT CAC ACA GGA CCC TCA TAG TTA GCG TAA CG ATT TAG GTG ACA CTA TAG TAA TAC GAC TCA CTA TAG GG TGC TAG TTA TTG CTC AGC GG ATT AAC CCT CAC TAA AGG GA GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG TAG AAG GCA CAG TCG AGG CGT GAC GCG GTG CAG GGC G TCT AAA AGC TGC GGA ATT GT pCMV-R pTRC99C-F: pTRC99C-R: pCEP-F: EBV-R : pIRES2-EGFP.P5’: pIRES2-EGFP.P3’: 3'AD: CMV -F S1 S6 5`AOX1 3`AOX1 α-Factor GAL4 AD pACT2-R pB42ADF: pB42ADR: pEGFP-N-5’: pEGFP-N-3’: pEGFP-C-5’: pEGFP-C-3' : PBV220F: PBV220R: U6 2.0rev primer: 3.0rev: S.tag 5'TriplEx2 RVP4 RVP3 pQE30-R pQE30-F pEF-F pDSRED-N1-R pDsRED-express-C1-F TCCAAACTCATCAATGTATC TTG CGC CGA CAT CAT AAC CTGCGTTCTGATTTAATCTG AGA GCT CGT TTA GTG AAC CG GTG GTT TGT CCA AAC TCA TC GTA GGC GTG TAC GGT GGG AG AAC GCA CAC CGG CCT TAT TC AGA TGG TGC ACG ATG CAC AG CGC AAA TGG GCG GTA GGC GTG CAA CGT GAA AAA ATT ATT ATT CGC GTA AAT GAA TTT TCT GTA GTA GG GAC TGG TTC CAA TTG ACA AGC GCA AAT GGC ATT CTG ACA TCC TAC TAT TGC CAG CAT TGC TGC TAC CAC TAC AAT GGA TG GTGCACGATGCACAGTTGAA CCA GCC TCT TGC TGA GTG GAG ATG AAG CCG ACA ACC TTG ATT GGA G TGG GAG GTC TAT ATA AGC AGA G CGT CGC CGT CCA GCT CGA CCA G CAT GGT CCT GCT GGA GTT CGT G TAT GGC TGA TTA TGA TCA GT AAG AAG GGC AGC ATT CAA AG CTG CGT TCT GAT TTA ATC TG ATG GAC TAT CAT ATG CTT ACC GTA AGG CGA TTA AGT TGG GTA GAG TTA GCT CAC TCA TTA GGC GAA CGC CAG CAC ATG GAC CTC CGA GAT CTG GAC GAG C GAC GAT AGT CAT GCC CCG CG CTA GCA AAA TAG GCT GTC CC GTT CTG AGG TCA TTA CTG G TGA GCG GAT AAC AAT TTC AC TCA AGC CTC AGA CAG TGG TTC TGA AGC GCA TGA ACT CCT TG TCC CAC AAC GAG GAC TAC AC pCMV5R pCMV5F P5' P3' BAC2 BAC1 3'BD: pTarget.F pTarget.R T7(17base) pYes2.R GAL4 BD pAS2-1.R pFlag-CMV-F: pFlag-CMV-R: pIRES-F: pIRES.R: P1 P2 pLEXA-F pLEXA-R pLNCX-F pLNCX-R pLXSN-F pLXSN-R pSHUTTLE-CMV-F pSHUTTLE-CMV-R MalE Primer ATT ATA GAG GAC ACC TAG TC TTC CAA AAT GTC GTA ATA AC TGC GTA CTG CGG TGA TCA AC CTG CAA GGC GAT TAA GTT GG ACG CAC AGA ATC TAG CGC TT AAC CAT CTC GCA AAT AAA TA TAA GAG TCA CTT TAA AAT TTG TAT AC CCA GGA TTT TCC CAG TCA C GGC TTT ACA CTT TAT GCT TC ACA TCC ACT TTG CCT TTC TC TCG GTT AGA GCG GAT GTG TCA TCG GAA GAG AGT AG AAG AGT TAC TCA AGA ACA AGA A GGT AGG CGT GTA CGG TGG GCA CTG GAG TGG CAA CTT CTT ACT GAC ATC CAC TTT GC CAC TGC ATT CTA GTT GTG GT CCA GGC TTT ACA CTT TAT GC GCG ATT AAG TTG GGT AAC GC CGT CAG CAG AGC TTC ACC AT TAA AAC CTA AGA GTC ACT TT AGC TCG TTT AGT GAA CCG TCA GAT CG ACC TAC AGG TGG GGT CTT TCA TTC CC CTT GAA CCT CCT CGT TCG AC GTT GCT GAC TAA TTG AGA TG GGT CTA TAT AAG CAG AGG TG GTG GTA TGG CTG ATT ATG ATC AG GGT CGT CAG ACT GTC GAT GAA GCC

引物名称 3’AOX 3'AD 3'BD 5’AOX1 A-FACTOR BAC1 BAC2 BGH rev 引物序列 5'-GGC AAA TGG CAT TC TGA CAT-3' 5'-AGA TGG TGC ACG ATG CAC AG-3' 5'-TAA GAG TCA CTT TAA AAT TTG TAT C-3' 5'-GAC TGG TTC CAA TTG ACA AGC-3' 5'-TAC TAT TGC CAG CAT TGC TGC-3' 5'-AAC CAT CTC GCA AAT AAA TA-3' 5'-ACG CAC AGA ATC TAG CGC TT-3' 5'-TAG AAG GCA CAG TCG AGG C-3' CMV-F CMV-R DsRed1-C-r DsRed1-N-f EBV Reverse EBV-R GAL4 AD-F GAL4 BD GLP1 GLP2 M13(-96) M13F M13F（-47） M13R M13R（-48） P24990 P31430 P12584 P17110 PAS2-1F PAS2-1R PB42ADF PB42ADR pbd-gal4-f pbd-gal4-r pbi-121(35s) PBV220F PBV220R PCDNA3.0-F2 PCDNA3.0-R2 PCDNA3.1F PCDNA3.1R pCEP Frorward PCEP-F PCMV-F PCMV-F PCMV-R PCMV-R PDONR-F PDONR-R PEF-F PEGFP-C-3’ PEGFP-C-5’ 5'-CGC AAA TGG GCG GTA GGC GTG-3' 5'-GTT CAC GGT GCC CTC C-3' 5'-AGC TGG ACA TCA CCT CCC ACA ACG-3' 5'-GTA CTG GAA CTG GGG GGA CAG-3' 5'-TGT GGT TTG TCC AAA CTC AAT C-3' 5'-GTG GTT TGT CCA AAC TCA TC-3' 5'-TAC CAC TAC AAT GGA TG-3' 5'-TCA TCG GAA GAG AGT AG-3' 5'-TGT ATC TTA TGG TAC TGT AAC TG-3' 5'-CTT TAT GTT TTT GGC GTC TTC CA-3' 5'-CCC TCA TAG TTA GCG TAA CG-3' 5'-TGT AAA ACG ACG GCC AGT-3' 5'-CGC CAG GGT TTTC CCA GTC ACG AC-3' 5'-CAG GAA ACA GCT ATG ACC-3' 5'-AGC GGA TAA CAA TTT CAC ACA GGA-3' 5'-TCATCGGAAGCGAGTAG-3' 5'-CGT TTT AAAACCTAAGAGTCAC-3' 5'-TTT TCA GTA TCT ACG AT-3’ 5'-TAC CAC TAC AAT GGA TG-3’ 5'-TCA TCG GAA GCG AGT AG-3' 5'-CGT TTT AAA ACC TAA GAG TCA C-3' 5'-CCA GCC TCT TGC TGA GTG GAG ATG-3' 5'-AAG CCG ACA ACC TTG ATT GGA G-3' 5'-GCC TCT AAC ATT GAG ACA GC-3' 5'-AAG AGT TAC TCA AGA ACA AGA A-3' 5'-GAC GCA CAA TCC CAC TAT CC-3' 5'-AAG AAG GGC AGC ATT CAA AG-3' 5'-CTG CGT TCT GAT TTA ATC TG-3' 5'-CTC TGG CTA ACT AGA GAA CC-3' 5'-GGC AAC TAG AAG GCA CAG TC-3' 5'-CTA GAG AAC CCA CTG CTT AC-3’ 5'-TAG AAG GCA CAG TCG AGG-3’ 5'-AGC AGA GCT CGT TTA GTG AAC CG-3' 5'-AGA GCT CGT TTA GTG AAC CG-3' 5'-TCT AAA AGC TGC GGA ATT GT-3' 5'-TTC CAA AAT GTC GTA ATA AC-3' 5'-TCC AAA CTC ATC AAT GTA TC-3' 5'-ATT ATA GAG GAC ACC TAG TC-3' 5'-TCG CGT TAA CGC TAG CAT GGA TCT C-3' 5'-GTA ACA TCA GAG ATT TTG AGA CAC-3’ 5'-TCA AGC CTC AGA CAG TGG TTC-3' 5'-TAT GGC TGA TTA TGA TCA GT-3' 5'-CAT GGT CCT GCT GGA GTT CGT G-3' PEGFP-N-3’ PEGFP-N-5’ pETup PGEX-3’ PGEX-5’ PIRES2-EGFP-F PIRES3-EGFP-R pMAL-C2X-F pMAL-C2X-R PQE30F PQE30R pTARGET-SEQ PTRC99C-F PTRC99C-R PTRCHISA-F PTRCHISA-R RVP3 RVP4 S.TAG S1 S6 SP6 T3 T7 T7（17BASE） T7TER TRXF TRXR malEfor pTrcHis-fw Xpressfor pTrcHis-rv N-CMV-30 C-CMV-24 CMV-Profor pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII) T7 EEV pDBLeu-5' pDBLeu-3' HGHrev PSG5(BAMHI)-F PSG5-R(SV40) 5'-CGT CGC CGT CCA GCT CGA CCA G-3' 5'-TGG GAG GTC TAT ATA AGC AGA G-3' 5'-ACG ATG CGT CCG GCG TAG AGG A-3' 5'-CCG GGA GCT GCA TGT GTC AGA GG-3' 5'-GGG CTG GCA AGC CAC GTT TGG TG-3' 5'-GTA GGC GTG TAC GGT GGG AG-3' 5'-AAC GCA CAC CGG CCT TAT TC-3' 5'-TGC GTA CTG CGG TGA TCA AC-3’ 5'-CTG CAA GGC GAT TAA GTT GG-3’ 5'-TGA GCG GAT AAC AAT TTC AC-3' 5'-GTT CTG AGG TCA TTA CTG G-3' 5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’ 5'-TTG CGC CGA CAT CAT AAC-3’ 5'-CTG CGT TCT GAT TTA ATC TG-3’ 5'-CAT GGT ATG GCT AGC ATG AC-3’ 5'-CAG GCT GAA AAT CTT CTC TC-3’ 5'-CTA GCA AAA TAG GCT GTC CC-3' 5'-GAC GAT AGT CAT GCC CCG CG-3' 5'-CGA ACG CCA GCA CAT GGA CA-3' 5'-CAA CGT GAA AAA ATT ATT ATT CGC-3’ 5'-GTA AAT GAA TTT TCT GTA TGA GG-3’ 5'-ATT TAG GTG ACA CTA TAG-3' 5'-ATT AAC CCT CAC TAA AGG GA-3' 5'-TAA TAC GAC TCA CTA TAG GG-3' 5'-ACA TCC ACT TTG CCT TTC TC-3' 5'-TGC TAG TTA TTG CTC AGC GG-3' 5'-CAT ATG AGC GAT AAA ATT ATT CAC-3' 5'-GGA TCC CTT GTC ATC GTC ATC ACC A-3' 5'-GGT CGT CAG ACT GTC GAT GAA GCC-3' 5'-TTA AAG AGG TAT ATA TTA ATG TAT CG-3' 5'-AGC ATG ACT GGT GGA CAG-3' 5'-ATC TGT ATC AGG CTG AAA ATC-3' 5'-ATA ACC CCG CCC CGT TG-3' 5'-TTA GGA CAA GGC TGG TGG-3' 5'-ATG GGC GGT AGG CGT G-3' 5'-CCA GGC TTT ACA CTT TAT GC-3' 5'-GCG ATT AAG TTG GGT AAC GC-3' 5'-ATG TCG TAA TAA CCC CGC CCC G-3' 5'-GAA TAA GTG CGA CAT CAT CAT C- 3’ 5'-CAG ATG GAA CGG TCT TTA AAT G-3’ 5'-CTG GGG AGG GGT CAC AGG G-3' 5'-GCT GGT TAT TGT GCT GTC TC-3' 5'-AAA CCA CAA CTA GAA TGC AGT-3' M13R(-26) pBRforBam pBRrevBam pBRforEco pBRrevHind c-mycrev pGAL4-ADrv pGAL4-BDrv pMD19T490R Psos5' Psos3' Primer TriplEx-LD-5´ Primer TriplEx-LDrv-3' UB-F 27F 1492R PTR5 pLXSN-F pLXSN-R pLEXA-F pLEXA-R pLNCX-F pLNCX-R pDSRED-N-R mtDNA-D-LoopF mtDNA-D-LoopR H1primer pRECEIVER-M03F pRECEIVER-M03R ACYCDuetUP1Primer #71178-3 DuetUP2 Primer DuetDOWN1 1525R pBAD_F pBAD_R AUG1F AUG1R V5rev EGFP-Nrev TKPAreverse pDsRED-express-C1-F DEST22F DEST22R 5'-CAG GAA ACA GCT ATG AC-3' 5'-CTT GGA GCC ACT ATC GAC-3' 5'-GGT GAT GTC GGC GAT ATA GG-3' 5'-AAT AGG CGT ATC ACG AGG C-3' 5'-GCG TTA GCA ATT TAA CTG TG-3' 5'-CTC TTC TGA GAT GAG TTT TTG-3' 5'-GAA CTT GCG GGG TTT TTC-3' 5'-AAT CAT AAG AAA TTC GCC C-3' 5'-CACAGGAAACAGCTATGACC-3' 5'-CCA AGA CCA GGT ACC ATG-3' 5'-GCC AGG GTT TTC CCA GT-3' 5'-CTC GGG AAG CGC GCC ATT GTG TTG G-3' 5'-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3' 5'-TCA GTG TTA GAC TAG TAA ATT-3' 5'-AGA GTT TGA TCC TGG CTC AG-3’ 5'-GGY TAC CTT GTT ACG ACT T-3’ 5'-CTC CGA GAT CTG GAC GAG C-3' CTT GAA CCT CCT CGT TCG AC GTT GCT GAC TAA TTG AGA TG CGT CAG CAG AGC TTC ACC AT TAA AAC CTA AGA GTC ACT TT AGC TCG TTT AGT GAA CCG TCA GAT CG ACC TAC AGG TGG GGT CTT TCA TTC CC TGA AGC GCA TGA ACT CCT TG AGG ACT Acg gct tga aaa gc atg tgc ctg acc gag gaa cca g CGCTATGTGTTCTGGGAAAT 5'-GCGGTAGGCGTGTACGGT-3' 5'-CCGGACACGCTGAACTTGT-3' GGATCTCGACGCTCTCCCT TTGTACACGGCCGCATAATC GATTATGCGGCCGTGTACAA 5'-AgAAAggAggTgATCcAgcc-3' ATGCCATAGCATTTTTATCC GATTTAATCTGTATCAGG 5'-CAATTTACATCTTTATTTATTAACG-3' 5'-GAAGAGAAAAACATTAGTTGGC-3' 5'-ATC GAG ACC GAG GAG AGG -3' 5'-CGT CGC CGT CCA GCT C-3' 5΄-CTTCCGTGTTTCAGTTAGC-3΄ TCC CAC AAC GAG GAC TAC AC 5’-tataacgcgtttggaatcact-3’ 5’-agccgacaaccttgattggagac-3’ pBMN_5' pYes2.R pSG5-3 pMSCV-5' pMSCV-3'

内参通用引物 primer 27F 515F 21F 312F 1492R 1525R ITS1 ITS4 gcttggatacacgccgc TCG GTT AGA GCG GAT GTG GAAATTTGTGATGCTATTGC CCCTTGAACCTCCTCGTTCGACC GAGACGTGCTACTTCCATTTGTC 16SDNAs sequence 5’-AGAGTTTGATCCTGGCTCAG-3’ 5’-GTGCCAGCMGCCGCGGTAA-3’ 5’-TTCCGGTTGATCCTGCCGGA-3’ 5’-TCCTACGGGAGGCAGCAGCT-3 5’-GGYTACCTTGTTACGACTT-3’ 5'-AgAAAggAggTgATCcAgcc-3' TCCGTAGGTGAACCTGCGG TCCTCCGCTTATTGATATGC 真菌通用引物？ nu-SSU-0817-5’ nu-SSU-1536-3’

TTAGCATGGAATAATRRAATAGGA ATTGCAATGCYCTATCCCCA