Methods for altering the cleavage specificity of a

专利名称：Methods for altering the cleavage specificity

of a type IIG restriction endonuclease

发明人：Shuang-yong Xu,Daniela Kobbe,Zhenyu

Zhu,James Samuelson

申请号：US10800946申请日：20040315

公开号：US20040219584A1公开日：20041104

摘要：Methods are provided for altering the cleavage specificity of a Type IIGrestriction endonuclease, the Type IIG restriction endonuclease being characterized by acleavage domain adjacent to a methylase domain, the methylase domain located adjacentto a specificity domain. The method includes ligating DNA or protein sequences to form afusion DNA or fusion protein. Where a fusion DNA is formed, the host cell is transformedwith the fusion DNA to express a Type IIG restriction endonuclease with altered cleavagespecificity.

申请人：XU SHUANG-YONG,KOBBE DANIELA,ZHU ZHENYU,SAMUELSON JAMES

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